A Purkinje neuron labeled with a voltage-sensitive dye shows spontaneous voltage and calcium ion concentration changes in the awake mouse.
A single Purkinje neuron was filled with ANNINE dye through a pipette at the cell body (top). A typical neuron has a cell body with many tree-shaped processes called dendrites, and a long cable-like process called the axon. The dendrites receive inputs, process information and pass it to the cell body. When a Purkinje neuron receives strong input, the entire dendrite gets activated (upstrokes in red trace) and the calcium ions flow into the neuron (transients in green trace). The voltage signals are 10-100 times faster than the variations in calcium levels.