GE Biacore T200 (Biacore)

Kinetics analysis applications.

IAS-A001

Kinetics analysis applications (protein – protein, protein – ligand)

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Features

Association rate (ka) (proteins) 10^3 to 3 × 10^9 M^-1s^-1

Dissociation rate (kd) 10^-5 to 1 s^-1

Affinity range     fM to mM

Concentration range 1 pM to 2 mM

Precision (concentration analysis) <5% CV

No molecular weight limit for organic molecules

Sample volume (kinetics)     50 to 400 μL

Immobilized molecule consumption 0.03 to 3 μg/flow cell

Analysis temperature 4°C to 45°C

Sample storage temperature 4°C to 45°C

Data collection rate 1 or 10 Hz

Sample capacity 1 × 96 or 384