onu FY2013 Annual Report 15

Figure 13: Quantification of Deep Cerebellar Nuclei (DCN) slice healthiness with multi-electrode array recordings. Left: representative recordings with 60 planar extracellular electrodes from 7-month old mice from DCN slices prepared at cold (top, blue) and warm (bottom, red) temperatures. Each grid square represents 1 second of continuous recording (verticalaxis: 100pA); blank squares are electrodes with degraded recording quality. Electrodes in which spiking cells were detected are indicated with black, thick squares. A micro graph combined with a schematic drawing of a coronal cerebellar slice (right top panel) shows positioning of the multi-electrode array within the DCN. Right, middle and bottom panels: Comparisons of average and cumulative sums of the numbers of spiking cells detected per slice in < +30◦C (+5 to 28◦C, blue) and +30 to +34◦C (red) conditions shows that when slices are prepared at warm temperatures, the probability of finding patchable neurons is increased. N = 23 (seven animals) slices cut in cold conditions, 10 (four animals) in warm.