mgu FY2016 Annual Report 3.3 fig4

Figure 4: Identification of all genes in the predicted biosynthetic pathway of sulfated fucans in the draft genome of Cladosiphon okamuranus. (A) A schematic representation of the biosynthetic pathway of sulphated fucans in brown algae, based on the description of Michel et al. Genes encoding each of the enzymes in the genomes of Cladosiphon okamuranus (C.ok) and Ectocarpus siliculosus (E.si), and the number of identified genes is shown in broken squares. (B) The location of candidate genes for l-fucosinase (FK) and GDP-fucose pyrophosphorylase (GFPP) in the C. okamuranus genome, suggesting the presence of an enzymatic gene cluster having shared synteny with E. siliculosus (http://bioinformatics.psb.ugent.be/orcae/overview/Ectsi). Two enzymatic genes that contain sulphotransferase and hydrolase domains are clustered with a gene (magenta) for a bifunctional enzyme with FK and GHMP-kinase domains. Genes coloured grey encode conserved proteins with RNA-binding domains.