SEM and STEM mode versus electron holographic imaging mode

16 Mar 2020

The two conventional modes of a scanning electron microscope (SEM and STEM; left and center) were unable to generate images of the biomolecules. However, holographic imaging mode (right), can be used to image biomolecules, such as the tobacco mosaic virus shown.

Modified from M Cheung, H Adaniya, C Cassidy, M Yamashita, T Shintake. Low-energy in-line electron holographic imaging of vitreous ice-embedded small biomolecules using a modified scanning electron microscope. Ultramicroscopy, 209 (2020) 112883, Fig 3.

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