SEM and STEM mode versus electron holographic imaging mode

16 Mar 2020

The two conventional modes of a scanning electron microscope (SEM and STEM; left and center) were unable to generate images of the biomolecules. However, holographic imaging mode (right), can be used to image biomolecules, such as the tobacco mosaic virus shown.

Credit: 
Modified from M Cheung, H Adaniya, C Cassidy, M Yamashita, T Shintake. Low-energy in-line electron holographic imaging of vitreous ice-embedded small biomolecules using a modified scanning electron microscope. Ultramicroscopy, 209 (2020) 112883, Fig 3.

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