"Genomic analyses of dosage compensation: from RNA polymerase recruitment to three-dimensional chromatin organisation" Juan M Vaquerizas (Max Planck Institute for Molecular Biomedicine)
Genomics and Regulatory Systems Unit (Luscombe Unit) would like to invite you to a seminar by Dr. Juan M Vaquerizase from Max Planck Institute for Molecular Biomedicine, Germany
Date: Tuesday, September 4th 2012
Time: 11:00 - 12:00
Venue: Seminar room C 209(Level C, Center Bldg.)
Title: Genomic analyses of dosage compensation: from RNA polymerase recruitment to three-dimensional chromatin organisation
Speaker: Dr Juan M Vaquerizas
Max Planck Institute for Molecular Biomedicine Roentgenstrasse 20, 48149 Muenster, Germany <http://www.mpi-muenster.mpg.de/en/research/teams/groups/rgvaquerizas/index.html>
Dosage compensation is a process that balances the expression of sex-linked genes in species that have evolved unequal numbers of sex chromosomes. In Drosophila, this involves hyperactivation of the single male X chromosome to equalise for the combined transcriptional activity of both female X chromosomes. The two-fold increase in expression is regulated by the MSL complex and involves extensive chromatin modiﬁcations and chromosomal organisation. Therefore, dosage compensation constitutes a prime example of epigenetic regulation. In this talk, I will present some of our latest results in the characterisation of this process. First, I will focus on a genomic analysis of the MSL complex and I will show how it regulates the recruitment of RNA polymerase II to expressed genes. Then, I will show how specific components of the MSL complex have evolved to regulate the expression of housekeeping genes. Finally, in the last part of the talk, I will focus on the three dimensional organisation of the chromatin in the interphase nucleus, which over the past years has become apparent to play a critical role in regulating transcription. I will show how, in Drosophila, nucleoporins act as a major class of global regulators of gene expression by establishing large domains of transcriptionally active chromatin. Our results suggest that nucleoporin binding could contribute to global chromatin organisation that could enable gene expression control.
- Conrad et al., (2012) Science, in press.
- Lam et al., (2012) PLoS Genet. 8:e1002736 Vaquerizas et al., (2010) PLoS Genet. 6:e1000846
We hope to see many of you at the seminar.
Research Administrator,Genomics and Regulatory Systems Unit